Part I The Basic Principles of Gene Cloning and DNA Analysis -- Why Gene Cloning and DNA Analysis are Important -- Vectors for Gene Cloning: Plasmids and Bacteriophages -- Purification of DNA from Living Cells -- Manipulation of Purified DNA -- Introduction of DNA into Living Cells -- Cloning Vectors for Escherichia coli -- Cloning Vectors for Eukaryotes -- How to Obtain a Clone of a Specific Gene -- The Polymerase Chain Reaction -- The Applications of Gene Cloning and DNA Analysis in Research -- Sequencing Genes and Genomes -- Studying Gene Expression and Function -- Studying Genomes -- The Applications of Gene Cloning and DNA Analysis in Biotechnology -- Production of Protein from Cloned Genes -- Gene Cloning and DNA Analysis in Medicine 269

15 Gene Cloning and DNA Analysis in Agriculture -- Gene Cloning and DNA Analysis in Forensic Science and Archaeology.